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Microbiological Safety Cabinets

Microbiological Safety Cabinets are used to provide protection to the operator, the environment, and the product while handling dangerous biological materials. It is important to differentiate between conventional Unidirectional Air Flow systems (formerly known as Laminar Air Flow or LAF) and Microbiological Safety Cabinets.

Unidirectional Air Flow systems provide a particulate-controlled atmosphere for critical operations but do not offer protection to the operator or the environment. In contrast, Microbiological Safety Cabinets are designed to ensure operator safety, environmental protection, and product protection.

The selection of a Biological Safety Cabinet depends on the type of biologically hazardous material handled inside the cabinet. Various international organizations have defined biological risk classifications based on the pathogenic nature of biological materials and the minimum safety requirements needed to protect personnel, the environment, and materials during handling.

Major organizations involved in developing pathogen classification and safety guidelines include the Dangerous Pathogens Advisory Group (DPAG) and the Department of Health and Social Security (DHSS), Great Britain; the Centers for Disease Control (CDC), USA; the World Health Organization (WHO); the National Cancer Institute (NCI), USA; and the National Institutes of Health (NIH), USA.

Classification of Microbiological Safety Cabinets

Different countries have adopted various standards and specifications for this equipment, but the following standards are generally accepted worldwide:

  • NSF/ANSI 49 -2002 (National Sanitation Foundation, USA)
  • EN 12469 -2000 (European Standard)
  • AS 2252 -2004 (Australian Standard)

Microbiological Safety Cabinets are divided into Basic 3 Classes

Function Class I Class II Class III
Operator protection Good Good Excellent
Environmental protection Good Excellent Excellent
Product protection None Excellent Poor

HyZone Class II Microbiological Safety Cabinets

HyZone Class II Microbiological Safety Cabinets from Thermolab blend knowledge, experience and innovative design changes in creating state – of – the – art equipment as part of its offerings. HyZone is a Class II Microbiological Cabinet, designed and built to meet, if not exceed, performance requirements of the EN 12469:2000 Standard. 70% air is re-circulated through the main H14 HEPA Filter within the cabinet, while the remaining 30% is exhausted through two H14 HEPA filters in series.
HyZone Cabinets are suitable for handling microorganisms and pathogens as defined by the appropriate International Standards, current health and safety guidelines and legislation aimed at safeguarding health and safety of operators at work. 95 % of your requirements are met by Class II A2 Cabinets.
However, Class I and Class III cabinets can be manufactured if your work so warrants.

HyZone Class II Cabinets are available in 3 types:
Class Exhaust Air % Recirculated Air % Exhaust Connection
II A2 30 70 Into Room/Loose Connected to exhaust duct
II B1 70 30 Ducted out of Room
II B2 100 0 Ducted out of Room
General Specification of HyZone Class II Microbiological Safety Cabinets:
Model A2003 A2004
Work table width 0.9 m (3′) 1.2 m (4′)
External Dimensions (W x D x H) Without Base Stand 1035 x 800 x1470 mm 1340 x 800 x 1470 mm
With Base Stand, 711mm 1035 x 800 x 2181 mm

982 x 563 x 670 mm

 1340 x 800 x 2181 mm

1270 x 563 x 670 mm
Internal Work Area Dimensions (W x D x H)
Average Airflow Velocity Inflow 0.45 m/s (90 FPM) +/- 20%
Downflow 0.30 to 0.45 m/s (60 to 90 FPM) with uniformity of better than +/- 20%
HEPA Filter Typical Efficiency Downflow >99.997% at 0.3 micron, H 14 as per EN 1822
Exhaust
Noise level Cabinet <62 dBA at 45 dBA ambient <62 dBA at 45 dBA ambient
Fluorescent Light Intensity >1200 Lux >1200 Lux

(>100 foot candles)
Cabinet Construction Main Body 1.2 mm (0.05″) 18 gauge steel with powder-coated finish 1.2 mm (0.05″) 18 gauge steel with powder-coated finish
Work table 1.5 mm (0.06″) 16 gauge stainless steel, type 304, 1.5 mm (0.06″) 16 gauge stainless steel, type 304,
Side Walls UV absorbing tempered glass, 5 mm (0.2″), transparent UV absorbing tempered glass, 5 mm (0.2″),transparent
Electrical 220-240V, AC, 50Hz, 1 Ф
Cabinet Power/ Amp 450 W / 2.5 AMP 500 W / 3 AMP

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